The flavonoids from lactuca tatarica were extracted by solvent⁃extraction method. The optimum extraction condition was determined by single factor experiment combined with the RSM. The results showed that the optimum extraction conditions for Mengshan lettuce were ethanol volume fraction of 70%, liquid⁃solid ratio of 40 mL/g, water bath temperature of 74 ℃, water bath time of 3.2 h. Under this condition, the actual yield of flavonoids was 37.06 mg/g (dry weight), which was very close to the prediction value. The model can simulate and forecast the yield well. This experiment provided the theoretical basis for actual production operation.